The use of restriction enzymes and electrophoresis to generate a map of the DNA molecule is known as RESTRICTION MAPPING. This technique is used to map an unknown fragment of DNA.
Restriction enzymes are enzymes that cut a DNA fragment at specific nucleotides sites, thereby generally smaller fragments of DNA.
Restriction mapping is a strategy used to map unknown fragments of DNA by digesting them into smaller fragments and then identifying the specific sites of the DNA breakpoints.
Restriction mapping can then be used to create a diagram of fragments of DNA marked with specific sites where they are cut by specific restriction enzymes.
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what percentage of extracellular fluid is accounted for by interstitial fluid and plasma?
Answer:
80%
Explanation:
You are attempting to carry out a transposon mutagenesis experiment using the pRL27 system. The Donor strain you use was giving to you by a lab mate who has been culturing it continuously for several months. When you set up your control plates, you find that the Donor strain grows poorly on Luria DAP Agar. Your transformation efficiency is very poor for this attempt at mutageneis. What likely happened to the Donor strain
The most likely happened to the donor strain is that the transposon is active in the donor resulting in the gradual accumulation of mutations. The accumulation of mutations is promoted by the insertion of the transposon.
A donor strain is a bacterial strain that contains a mobilizable fragment of DNA called plasmid.
These strains (donor strains) exploit the transference properties of a conjugative plasmid to modify a particular recipient strain.
Transposons, also known as jumping genes, are mobile genetic elements that alter the genome of an organism by jumping and thus inserting them into genetic sequences (i.e., into protein-coding sequences).
In this case, the presence of a transposon in the donor strain affected the expression of many genes required for the donor strain in order to grow into Luria DAP Agar.
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What is the main purpose for plants to conduct photosynthesis?
what insects don't have piercing and sucking mouth parts?
.
Answer:
lice
Explanation:
they bite and chew on their host skin
What are valence electrons?
Answer: : a single electron or one of two or more electrons in the outer shell of an atom that is responsible for the chemical properties of the atom.
Explanation:
Adaptational characteristics of himalayan animals
Answer:
Thick layer of body fat or bubbler, to reduce body heat loss. Strong hooves for walking on rock slopes. Thick fur on paws for protection from cold while walking on snow.
Explanation:
1) Thick layer of body fat or bubbler, to reduce body heat loss.
2) Strong hooves for walking on rock slopes.
3) Thick fur on paws for protection from cold while walking on snow.
Which is the odd
one? Explain.
A. RR
B. Rr
C. Aa
D. aa
Answer:
d aa its not capitalized like the other ones
Explanation:
Which structure gives you voluntary control over urination?
Answer:
d. External urethral sphincter
Explanation: is correct. post protected
in response to action potentials arriving along the transverse tubules, the sarcoplasmic reticulum releases:
7)
.......... and....
Are weak forces of attraction.
Answer:
inter molecular forces and van der wall forces are weak forces of attraction.
Explanation:
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What are the differences between an onion and a whitefish cell
Which part of the plant cell does photosynthesis occur? Please help
Answer:
Photosynthesis occurs in the chloroplast of the plant cell.
Explanation:
what gives animal cells their shape and structure?
Answer: Animal cell's shape and structure are determined by the cytoskeleton.
What is the cytoskeleton?
The cytoskeleton is present in all animal cells, prokaryotic (bacterial) cells contain a very rudimentary version of it, while plant cells do not have one at all. It consists of several types of large molecules that provide physical support to the cell as well as internal transport along its length for organelles like endoplasmic reticulum (ER), mitochondria, vacuoles, etc. The three main components are microtubules, intermediate filaments and microfilaments which give rise to different structures within the cell. Microtubules are long cylindrical tubes made up of two protein subunits (alpha and beta tubulin) that form a hollow tube. These are the main components of the cytoskeleton, they provide support to the cell while also having transport capacity for organelles like endoplasmic reticulum (ER), mitochondria, vacuoles, etc. Microtubules are dynamic structures which means their state is constantly changing i.e., growing, shrinking or moving around depending on requirements of the cell.
Please help me!! ASAP
Answer:
B) Mushroom --> Heterotroph
C) Algae --> Autotroph
A) Autotroph --> Producer
D) Hetrotroph --> Comsumer
Explanation:
Mushrooms are decomposers because like other fungi, they break down dead and decaying matter to make their own foodProducers, such as plants and algae, acquire nutrients from inorganic sources that are supplied primarily by decomposers whereas decomposers, mostly fungi and bacteria, acquire carbon from organic sources that are supplied primarily by producers.Primary producer; organisms, such as plants and phytoplankton, that can produce their own food through photosynthesis or chemosynthesis; also called autotrophs.Consumers are also referred to as heterotrophs in contrast to autotrophs, which are the producers of the food chain. Consumers therefore include animals and heterotrophic bacteria and fungi. Plants that are carnivorous and therefore consume organic matter as well are regarded as both consumers and producers.Have a great day! :D
In angiosperm double fertilization, one sperm nucleus fertilizes the ______ to form the diploid ______, and a second sperm nucleus fuses with the two nuclei in the central cell of the embryo sac.
Answer:
one sperm fertilizes the egg cell, forming a diploid zygote
Explanation:
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In angiosperm double fertilization, one sperm nucleus fertilizes the egg to form the diploid zygote, and a second sperm nucleus fuses with the two nuclei in the central cell of the embryo sac.
The egg is the female gamete in an angiosperm. It is a haploid cell that contains one set of chromosomes. The zygote is the fertilized egg. It is a diploid cell that contains two sets of chromosomes. The zygote will eventually develop into an embryo. The central cell is a cell in the embryo sac that contains two haploid nuclei.
Double fertilization is a unique feature of angiosperms. It is a type of fertilization in which two sperm nuclei fertilize two different cells in the embryo sac. The first sperm nucleus fertilizes the egg to form the zygote. The second sperm nucleus fuses with the two nuclei in the central cell to form a triploid cell. This triploid cell will eventually develop into the endosperm, which is a food reserve for the developing embryo.
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Photosynthesis (Where does the electron that is excited by light is taken). What is the source of the electron, where does it end up?
Answer:
The source of the light electron is the sun and it is taken in by the chlorophyll contained in the chloroplasts.
Explanation:
As a part of her homework, June make a flowchart about the steps involved in hearing the sound energy produced by wind chimes.
A flowchart with four steps. The first step is the rods vibrate back and forth. The second step is wind chime rods hit each other. The third step is sound waves are created. The fourth step is the sound travels into your ears.
Which statement best explains the error in June's flowchart?
The wind chime rods hit each other before they begin to vibrate.
Sound waves are created before the wind chime rods hit each other.
The sound of the wind chimes travels to your ear before the rods vibrate.
The spin of the rods causes sound energy, not its vibrations.
Answer:
A is the final answer........
different types of cells in your body look and function differently. the basis for these differences is:
Different types of cells look and function differently, the basis for these differences is DIFFERENT PATTERNS of gene expression. This process (differential gene expression) leads to cell specialization.
Gene expression refers to the process by which the genetic information flows from a DNA sequence (i.e., a gene) to an mRNA molecule and finally to a protein.
In somatic (body) cells, the differential patterns of gene expression lead to the generation of different proteins and ultimately to different types of cells (cell phenotypes).
In consequence, cells are specialized to perform specific functions because different genes are being expressed.
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. A kidney cell is an example of which type of cell?
a. somatic cell
b. germ cell
c. sex cell
d. haploid cell
Answer:
A. Somatic cell
Have a good day!!!
Explanation:
High-energy electrons from NADH and FADH2, are passed into and along the____ The energy from the electrons moving down the chain is used to move H+ ions across the_____ H+ ions build up in the_____ space, making it_____ charged and making the matrix negatively charged. in the inner membrane. H+ ions move through channels of______ The ATP synthase uses the energy from the moving ions to combine ADP and phosphate, forming high-energy______
Answers:
High-energy electrons from NADH and FADH2, are passed into and along the electron transport chain .
The energy from the electrons moving down the chain is used to move H+ ions across the inner membrane .
H+ ions build up in the intermembrane space, making it positively charged and making the matrix negatively charged.
In the inner membrane, H+ ions move through channels of ATP synthase .
The ATP synthase uses the energy from the moving ions to combine ADP and phosphate, forming high-energy ATP .
Discuss 2 other ways that scientists believe
kingdoms should be divided.
Answer:
Plants and animals? ...The question says two OTHER ways, and I don't know what ways have already been listed.
Explanation:
The two main kingdoms in the animal kingdom are plants and animals.
Scientists also list four other kingdoms including bacteria, archaebacteria, fungi, and protozoa.
Hope this helps answer the question. Comment if you need further help!
lymph is largely composed of water that has escaped from
they overcame by the blood of the lamb and the word of their testimony
Answer:
Revelation 12:11
what describes metal for being malleable?
Malleable metals will bend and twist into numerous shapes when affected by a hammer, whereas non-malleable metals might break apart into pieces. Examples of malleable metals are gold, iron, aluminum, copper, silver and lead. ... No metal is completely malleable, and all will break when under extreme stress
Question 41 A basement membrane anchors Multiple choice question. A) muscle tissue to nervous tissue. B) epithelial tissue to connective tissue. C) connective tissue to muscle tissue. D) brain tissue to nervous tissue. E) blood cells to plasma.
Answer:
B) Epithelial tissue to connective tissue
Explanation:
Which layer of the atmosphere is experiencing this storm?
Answer:
Troposphere
Explanation:
Almost all weather occurs in the lowest layer of the atmosphere, called the Troposphere. The exception being large storms like hurricanes and large Thunderstorms. These storms have clouds with great vertical development and the tops of these storms go into the Stratosphere.
4. Arterioles are the
a. Vein system.
b. Arteries system.
c. Arteries and vein system.
d. Arteries and blood system
Answer:
b. Arteries system
Explanation:
who has a German shepherd? I want a dog and need some information form a dog owner.Plz help
Answer:
That is the type of dog that police use to find drugs, look for people, etc.
Explanation:
Choose the correct response to this statement: Presynaptic neurons synapse on the ________ of the postsynaptic neuron.
Answer:
Cell or body dendrites
Explanation:
The cell which connects the whole body is called neuron cell and the tissue made up from the following is known as nervous tissue.
The correct answer to the following is left.
The synapse is made of the following:-
Cell endingDendriteThe signal transfer to the whole body is in form of electrical and chemical signals.
Hence, the correct answer is left to the postsynaptic neuron.
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A DNA microarray contains immobilized DNA that corresponds to different genes. The microarray is hybridized with fluorescently labeled DNA probes reverse transcribed from the control and experimental mRNA samples. The control probe is labeled with a red fluorochrome, while the experimental probe is labeled with a green fluorochrome. What will be the color of the spot representing a gene with moderately upregulated expression in the experimental sample relative to the control one
The color of the spot of a gene with moderately upregulated expression will be lime-green. A DNA microarray is a technique used to determine the expression level of many genes simultaneously.
A DNA microarray is a laboratory technique used to identify the genes that are being expressed in a cell.
A DNA microarray contains many tiny spots of specifically labeled cDNA samples referred to as probes, which can be hybridized by base complementarity to transcribed (mRNA) gene sequences.
In the DNA microarray, the probes can be fluorescently marked gene sequences.
After hybridization, the DNA microarray is then scanned in order to determine the level of gene expression of each mRNA that hybridizes with their corresponding probes on the slide.
In this case, green spots evidence genes that are being strongly expressed in the sample.
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