Often in laboratory experiments you will want to amplify regions of the genome from several different preparations of gDNA. To do this, a mastermix will be created with all the common reagents to ensure easier pipetting, proper mixing, and controlling for variations in pipetting. Create a mastermix for running 8 identical reactions, including one negative control, each with different gDNA templates (7 total gDNA templates).

Answers

Answer 1

Answer:

Mastermix in PCR helps in providing faster and easier setup with pipetting, proper mixing, reduction in contamination and controlling fir variation in pipeting or tube to tube variability. Contents of PCR master mix for the desired reaction are as follows:

dNTPsPrimersthermostable DNA polymerasecofactor (MgCl2)Template DNA (for 7 samples)PCR-grade water

The only difference in negative control for the PCR master mix is template or the different primer rest the components are the same in the master mix.


Related Questions

The graphs show the reaction rate for an enzyme across a range of temperatures and pH. Based on these data, this enzyme functions best at what temperature and pH? *

Answers

The graph is attacged and the options is as follows:

Temperature of 27oC and a pH of 4

Temperature of 37oC and a pH of 6

Temperature of 40oC and a pH of 8

Temperature of 50oC and a pH of 10

Answer:

The correct answer would be - temperature of 37oC and a pH of 6

Explanation:

The graph 1 shows the relation between the rate of reaction for an enzyme and the temperature of  reaction is been represented. In this graph the rate of eaction is highest (point from the slope starts decreasing) at the So, by the options and graph the temperature 37°C(Below 40°C) rate of reaction is maximum.

Similarly, in other graph rate of reaction and and pH has been represented in which rate of reaction is maximum at pH 6. after 6 pH the rate of reaction started decreasing.

Carbohydrates like glucose can undergo spontaneous rearrangement to give an alternate configuration for the anomeric carbon when the ring is free in solution. However, when glucose is covalently attached to another sugar through C1, this rearrangement cannot occur. Consequently, the configuration of carbohydrate oligomers and polymers is fixed. Why can't glucose rearrange when in a polymer form?

Answers

Answer:

The presentation according to another circumstance is categorized in the following section.

Explanation:

Glucose constructs anomers because of the extreme involvement of flexible metal-carbon because that continues to exist as something of a monomer. However, when plastics are established, transformation is limited with glycoside formation of complexes among both anomeric carbon atoms.

Sucrose, besides obvious reasons, would be manufactured by glucose and fructose, which have been connected by something like a glycerol backbone involving glucose carbon atom as well as fructose. The mechanism including its creation of anomers even by movement through carbon becomes called "Mutarotation" Sucrose doesn't always show mutation.

What two factors play a role in the type of snow flake created?

Answers

Answer:

Two factors play key roles in determining the shape of a snowflake: temperature and humidity.

Explanation:

According to scientists, all of the bodies in the universe came from a rotating cloud of gases and dust about
billion years ago.

O 3.5
O 2.5
O 4.5
O 1.2

Answers

It's either 3.5 or 1.2

There would be more evidence spread out through space to be noticed by scientists and it seems like a stretch to go as far back as 4 billion years ago.

(I mean really? That much before the DINOSOURS died? I don't think so)

◕‿◕

Hope this helps

Miri

Match the term to its definition.


control group, data, hypothesis, inference

observations that may be quantitative or qualitative


a tentative, testable scientific explanation


useful for determining the effect of the independent variable


a logical interpretation based on prior knowledge

Answers

Explanation:

hypothesis is the last one

data is the 3rd one

inference is the 2nd one

and control group is the first one

process of elimination is your best friend

Study the diagram of a cell.
Which structures are found in both plant and animal
cells?
O W and X
O W and Z
O X and Y
Y and Z

Help me please

Answers

if this is the diagram.

the answer would be W and Z

There is a multitude of evidence that supports how life has changed over time due to variation in the gene pool of different species. Which of the following choices below represents a scientific theory that explains these changes? *
A. As environments and ecosystems change over time, those that acquire suitable traits are more likely to survive and reproduce and the gene pool changes because organisms must adapt to survive and reproduce.
B. As environments and ecosystems change over time, those with variations for traits that are more successful in the new conditions are more likely to survive and reproduce, thus changing the allele frequency in the gene pool.
C. Because the environments and ecosystems show little change over time, organisms are already adapted for surviving in their given habitats for which they are made.
D. Because the environments and ecosystem show little change of time, the organisms that choose to adapt to different environments will migrate and become suited for new habitats.

Answers

Answer: A. Environmental drivers plus genetic variation in the population result in natural selection

Explanation: over geological time environmental conditions change. Organisms do not survive in one environmental niche with traits adapted to a possible future change that might suit those traits. Adaptation is not a matter of choice: adapt if possible or go extinct.

There is a (badly named) concept called pre-adaptation, which is that variations in some existing trait render adaptation to a different environment more likely.

Answer:

A

Explanation:

Please please please please help me please

Answers

Answer:

A. Bees and Butterflies receive food while pollinating the flowers.

Your answer is A. Bees and butterflies receive food while pollinating the flowers

The diagram below shows the morphological change of two species.
Which statement explains why species 1 and species 2 are different?

Answers

Natural selection can cause gradual speciation changes.

Natural selection favors traits that can transmit through generation.It can leads to a new distinctly different species.

What is natural selection?

It is the process through which populations of living organisms adapt and change to new environment conditions and pass these genes to next generations.

Define speciation?It is how a new species of an organism is created.It occurs when a group within a species separates from other members of its species and develop its own unique characteristics.

To know more about natural selection here

https://brainly.com/question/9830102

#SPJ2

What is the danger posed by introduced species? *
O Non-native species might have no natural predators in their new environment
Non-native species can occupy space and crowd out indigenous species
Non-native species might prey on native species threatening extinction
All of these are dangers posed by introduced species

Answers

all of these are dangers posed by introduced species

Choose all the answers that apply.
Ground tissue
stores extra food
supports the plant
is found in plants and animals
Omakes up the majority of a plant
Dis responsible for photosynthesis

Answers

Answer:

stores extra food

is responsible for photosynthesis

supports the plant

makes up the majority of a plant

Explanation:

Ground tissues are one of the three types of plant tissues (others being dermal and vascular). They are composed of cells as follows: parenchyma, collenchyma, and sclerenchyma. These three cells that make up the ground tissues perform different functions in the plant cell.

- Parenchyma functions in photosynthesis and storage of extra food (carbohydrates) in the cell.

- Collenchyma functions in the support of young regions of the plant

- Sclerenchyma, which has the thickest cell wall, functions mainly in the strengthening/support of the plant cell.

Also, among the three tissue types in a plant, ground tissues make up majority of the plant cell.

Which best describes the process of evaporation/transpiration in the
water cycle?
O A. Water from the ocean enters the atmosphere as water vapor.
O B. Water on the surface of Earth enters the groundwater supply.
C. Water vapor in the atmosphere returns to the surface of Earth as
liquid water.
O D. Water on the surface of Earth and water lost by plants enters the
atmosphere as water vapor.

Answers

Answer:

A

Explanation:

Answer:

The answer is D

Explanation:

Water on the surface of Earth and water lost by plants enters the atmosphere as water vapor

The person above me is wrong I tried it and it was wrong and I tried D and I got it correct

3. What things does your body do automatically to cool down or warm up?

Answers

Answer:

For your body to cool down one method is to sweat.

Explanation: Your sweat glands release sweat, which cools your skin as it evaporates. This helps lower your internal temperature

To keep warm your body shivers and your blood pressure decrease, it brings the blood back to the heart to keep you warm, also in you blood, your hormones create glucungons that makes the liver shoot out glucose to keep you warm. To keep cool you body sweats and in you blood the hormones create something called insulin that makes you cell take in the glucose in you blood.(Fun Fact: the things that I just said are negative feedback).

In humans, mitosis produces cells with ____ chromosomes and _____ chromosomes are found in the gametes after Meiosis.

Answers

Answer:

46, 23

Explanation:

PLEASE HELP ME ITS FOR 17 POINTS!!!!!!!!!!!!!!!!!!!!

A forest area is being used for a new housing development. Most of the trees have been removed to make room for the
new houses. Predict the impact that this will have on the birds in the area.
The bird populations will stay the same because humans love birds.
The bird populations will decrease because humans see them as pests.
© The bird populations will increase because humans do not interfere with wild animals.
The bird populations will decrease due to competition for resources such as food and nesting sites.

Answers

Answer:

The bird populations will decrease due to competition for resources such as food and nesting sites.

Explanation:

Their food and habit is being taken away so they will have to compete. I hope this helps you! :)

Possible Brainliest? You don't have to if you don't want to!

1) Organisms reproduce either sexually or asexually. Asexual
reproduction is the reproductive process in which offspring have only
one parent. Sexual reproduction involves two parents who combine
their genetic material to produce a new offspring. Which of the
pictures show asexual reproduction
A) A&B
B) B&C
C) C&D
D) A,B, & C

Answers

Answer: NO BLOODY PICTURE. I never get the picture, diagram whatever, so why do I get the question?

Explanation:

I NEED HELP PLEASE!!!

Answers

Answer:

I believe it is convergent boundaries

Explanation:

Answer:

C convergent boundaries

How is Gypsy’s story related to psychology?

Answers

Answer:

Gypsy’s story is directly related to psychology.

Explanation:

Gypsy’s story is related to psychology because according to her mother who said that her daughter Gypsy Rose suffering from leukemia, and mental disorder but this claim of her mother proved wrong when the full health check up of Gypsy Rose was held. The doctors said that she was psychologically torture by her mother which leads to the killing of her mother.

Brainliest Which of the following best explains how most plants grow? *


Plants store the excess oxygen they produce, resulting in them gaining mass

Plants store the excess glucose they produce, resulting in them gaining mass

Plants store the excess carbon dioxide they produce, resulting in them gaining mass

Plants store the excess water they produce, resulting in them gaining mass

Answers

Answer:

plants store the glucose they produce, resulting in them gaining mass. that should be the correct answer

I need help. Thanks!

Answers

Answer:

They bounce around and take form of the container

Explanation:

C6H12O6 + 6O2 --> 6H2O + 6H2O]\


Is the equation above balanced?



No; there are un equal numbers of each type of atom on both sides.


No; the mass of reactants and products do not equal one another.


Yes; it has equal numbers of each type of atom on both sides.


Yes; the mass of the reactants are equal.

Answers

Answer:

no

Explanation:

the carbon isnt there at all

Other Questions
Please help. Ill give brainliest :) The term carcinogen refers to:the spread of disease.the chemicals used to stop the spread of cancer.a term that means the same as an aneurysm.a substance that produces cancer. What was the goal of the Moscoso expedition?a.to find Cibolab.to find gold and riches in Floridac.to find an overland route to New Spain (Mexico)d.to explore the Southeastern part of what is now AmericaPlease select the best answer from the choices providedABCD In your own words, explain the benefits and limitations of using submarines inWWI. EscogerSelect the correct option between verbs saber and conocer.Mis hermanos __________ bailar salsa.conocensaben what is the central idea of One Last Time by Gary Soto? Identify the parts of a good hypothesis? Please explain how to do this please show work HELP MY LITTLE SISTER IS TAKING A TEST AND THIS MAKES NO SENSE Which word has a syllable (word part) that is related to Greek mythology?manictunictitanicbasic Use the words you're, their and its with no apostrophe in a sentence Which part of the election process helps political parties pick their candidates for office? You are attempting to convince your class that they shouldnt use vape pens Cover pages should include a. The name of your class c. Your teachers name b. The date you turn in the paper d. All of these Please select the best answer from the choices provided A B C D Mar Alejandro uses the steps below to convert StartFraction 5 over 8 EndFraction to a percent. Five-eighths right arrow 8.0 divided by 5 = 1.6. 1.6 = 160 percent. Which best explains Alejandros error? The division was computed incorrectly. The division was completed in the incorrect order. The decimal was moved to the right too many times. The decimal was moved to the right instead of the left. When bonds are broken between atoms in molecules, energy isa. stored as ADPb. releasedc. createdd. conserved An individual who is relatively good at transformational coping has _____. a. overdependent behavior.b. a Type A personality.c. low control.d. a hardy personality. "The output is 5 less than twice the input Which of the following are two-step equations? Choose more thanone answers Click on photo if neccessary i am a decimal number less than 1. the digit in my tenths place is an even prime number. my hundredths digit is twice my tenths digit, and my hundred thousandths digit is twice my hundredths digit. the thousandths digit is three less than my hundredths digit. my ten thousandths digit is the sum of my tenths and thousandths digit. what number am i? A and B are independent events.P(A) = 0.6P(B) = 0.4What is P(AB)?O A. 0.24O B. 0.6O C. 0.4D. Not enough information