A binding protein binds to a ligand L with a Kd of 400 nM. What is the concentration of ligand when Y is (a) 0.25, (b) 0.6, (c) 0.95?

Answers: (a) 133.33nm (b) 600nm (c) 7,600nm

Answers above are correct. Please show all steps, really trying to understand.

Answers

Answer 1

Answer:

(a) 133.33nm

(b) 600nm

(c) 7,600nm

Explanation:

The concentration of Y can be determined by using the formula:

[tex]Y = \dfrac{[L]}{k_d+[L]}[/tex]

where;

[L] = concentration of the binding ligand.

kd = 400 nm

Thus:

When Y = 0.25; we get :

[tex]0.25 = \dfrac{[L]}{400+[L]}[/tex]

0.25 (400 + [L]) = [L]

100 + 0.25[L] = [L]

100 = [L] - 0.25 [L]

100 = 0.75 [L]

[L] = 100/0.75

[L] = 133.33 nm

At, Y = 0.6

[tex]0.6 = \dfrac{[L]}{400+[L]}[/tex]

0.6 (400 + [L]) = [L]

240 + 0.6[L] = [L]

240 = [L] - 0.6 [L]

240 = 0.4 [L]

[L] = 240/0.4

[L] = 600 nm

At, Y = 0.95

[tex]0.95 = \dfrac{[L]}{400+[L]}[/tex]

0.95 (400 + [L]) = [L]

380 + 0.95[L] = [L]

380 = [L] - 0.95 [L]

380 = 0.05 [L]

[L] = 380/0.05

[L] = 7600 nm


Related Questions

how many moles of nitroge are there in 50.0 g of nitrogen?

Answers

1.785714286 moles

The number of moles (n) for nitrogen is: [ n=50.0÷28.0 ] = 1.785714286 moles.

Write the overall molecular equation for the reaction of hydroiodic acid ( HI ) and potassium hydroxide. Include physical states. Enter the formula for water as H2O .

Answers

Answer:

HI(aq) + KOH(aq) --> KI(aq) + H2O(l)

Explanation:

HI is an acid and KOH is a base, this means that the reaction would be a neutralization reaction.

The equation is given as;

HI + KOH --> KI + H2O

Adding the physical states we have;

HI(aq) + KOH(aq) --> KI(aq) + H2O(l)

state Graham's law diffusion of gas mathematical expression ?​

Answers

Answer:

Graham's law states that the rate of diffusion or of effusion of a gas is inversely proportional to the square root of its molecular weight

Explanation:

What is the pH value of distilled water?

Answers

Answer:

7

Explanation:

The ph level should be 7, but because it absorbs carbon dioxide from the atmosphere it’s actually slightly acidic with a pH of 5.8

Sulfur has an electronegativity of 2.58, while Cesium has a value of 0.79. Based on this information, they would have what kind of chemical bond?

Answers

Answer:

Ionic

Explanation:

Because sulphur is a non-metal and cesium is part of the group 1 Alkali Metals, this creates an ionic bond

Ionic bonds are created from one metal and one non-metal element.

This still is declared as Ionic despite the balance of this is Cs2S

Based on your lab report, provide the chemical reaction that occurs in Benedict's test for the following molecule.​

Answers

Explanation:

I DONT UNDERSTAND WHAT U WANT TO SAY

You have two containers. The first container contains 22.99 grams of solid
sodium (Na). The second container contains 15.999 grams of Oxygen (O).
Which container has more moles of the indicated substance? *

Answers

Answer:

The sodium has more moles.

n(Na)=2.09 mole

n(O)=0.999 mole

As you move from N to Bi, the metallic properties of the element
A. increases.
C. remains the same.
B. decreases.
D. does not follow a trend.

Answers

Answer:

b. decreases

Explanation:

i just took a test on it

As you move from N to Bi, the metallic properties of the element decrease. The correct option is B.

What are nitrogen and bismuth?

Nitrogen is a chemical element, whose chemical element is N. Nitrogen is a gas and its atomic number is 7. It is a non-metal and is present in group 15 of the periodic table.

Bismuth is a chemical element, whose chemical element is Bi. It is a post-transition metal, and it is also present in group 15 of the periodic table near arsenic and antimony. Its atomic number is 83.

Now, nitrogen and bismuth are present in the same line and group. Nitrogen is the first element of the group and going down, there is bismuth present in the group last. So the metallic property decreases from going down the group.

Thus, the correct option is B. decreases.

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Please help I beg you

Answers

Answer:

is highly respect is correct I promise!

Which statement accurate describes plate tectonics?

Answers

Answer:

the lithosphere is broken into sections called plates.

Answer:

The lithosphere is broken into sections called plates.

Explanation:

edge2020

pls help!!

The number that represents a neutral pH is ________.​

Answers

Answer:

7

Explanation:

7 is the number that represents a neutral pH

Is steel wool and vinegar an exothermic reaction? Explain.

Answers

Answer:

Vinegar contains acetic acid which removes this coating. When the protective coating is removed, oxygen in the atmosphere can reach the iron in the steel wool and a chemical reaction called oxidation occurs. ... This reaction is also an exothermic reaction. It creates heat energy

Explanation:

hope this helps u

Vinegar incorporates acetic acid which gets rid of this coating. When the defensive coating is removed, oxygen in the environment can reach the iron in the metal wool and a chemical reaction called oxidation occurs. This reaction is additionally an exothermic reaction. It creates warmth energy.  

Hope it helped! Have a nice day! <3

5. How many moles of aluminum sulfate would contain 3.54 x 1025 sulfur atoms?

Answers

Answer:

19.6 moles of aluminum sulfate

Explanation:

Given parameters:

Number of sulfur atoms  = 3.54 x 10²⁵  atoms

Unknown:

Number of moles of aluminum sulfate = ?

Solution:

Aluminum sulfate is expressed as;

        Al₂(SO₄)₃

  1 mole of Aluminum sulfate contains 3 moles of sulfur.

 

 6.02 x 10²³ atoms are found in 1 mole of any substance;

 3.54 x 10²⁵  sulfur atoms will be found in [tex]\frac{3.54 x 10^{25} }{6.02 x 10^{23} }[/tex]   =  58.8moles of sulfur

1 mole of Aluminum sulfate contains 3 moles of sulfur.

 or ;

      3 moles of sulfur is contained in 1 mole of aluminum sulfate

     58.8moles of sulfur will contain [tex]\frac{58.8}{3}[/tex] moles of aluminum sulfate

                                                       = 19.6 moles of aluminum sulfate

what are the reactants in a cheese sandwich

Answers

Answer:

cheese and bread

Explanation:

2 bread 1 cheese = cheese sandwich

Cheese and Bread
Explanation:

3. What is the pairing arrangement of the nitrogenous bases? _____________ pairs with __________ and ____________ pairs with ___________.

Answers

Answer:

Adenine pairs with thymine and guanine pairs with cytosine

A very new item just arrived in the market. It is very good for construction, and other things. Now, this item has chemicals, and in every drop, there are 400 grams of NaCI. If there are 10 drops in this item, how many grams of NaCI are in one bottle?

Answers

Answer: 4,000 grams of NaCI

Explanation:

We know that there are 400g of NaCI in one drop.

This bottle has 10 drops of this item.

So, we have to multiply the amount of NaCI in a drop by the amount of drops.

400 x 10 = 4,000.

The answer to the question is 4,000. Hope this helps!

3.0 x 108 m/s is the

Answers

Answer:

Speed of light

Explanation:

The value 3.0 x 10⁸m/s is taken as the speed of light.

It is a constant.

It implies that light travels a distance of 3 x 10⁸ in just one second. This value is for the speed of light in a vacuum when there are not particles obstructing its movement. The speed of electromagnetic radiations in free space is also taken as the speed of light.

WILL GIVE 50 POINTS AND BRAINLIEST
Plate Tectonics Lab Report
Instructions: In the Plate Tectonics lab you will investigate the interactions between continental and oceanic plates at convergent, divergent, and transform boundaries around the globe. Record your observations in the lab report below. You will submit your completed report.

Name and Title:
Include your name, instructor's name, date, and name of lab.


Objective(s):
In your own words, what was the purpose of this lab?


Hypothesis:
In this section, please include the if/then statements you developed during your lab activity for each location on the map. These statements reflect your predicted outcomes for the experiment.


Location One: Select two events that you predict will be observed. If I explore two continental plates at a convergent boundary, then I will observe:

earthquakes
faults
ocean formation
mountains
volcanoes
island chains
seafloor spreading
Location Two: Select three events that you predict will be observed. If I explore two continental plates at a divergent boundary, then I will observe:

earthquakes
faults
ocean formation
mountains
volcanoes
island chains
seafloor spreading
Location Three: Select three events that you predict will be observed. If I explore two continental plates at a transform boundary, then I will observe:

earthquakes
faults
ocean formation
mountains
volcanoes
island chains
seafloor spreading
Location Four: Select two events that you predict will be observed. If I explore two oceanic plates at a convergent boundary, then I will observe:

earthquakes
faults
ocean formation
mountains
volcanoes
island chains
seafloor spreading
Location Five: Select three events that you predict will be observed. If I explore two oceanic plates at a divergent boundary, then I will observe:

earthquakes
faults
ocean formation
mountains
volcanoes
island chains
seafloor spreading
Location Six: Select two events that you predict will be observed. If I explore two oceanic plates at a transform boundary, then I will observe:

earthquakes
faults
ocean formation
mountains
volcanoes
island chains
seafloor spreading

Procedure:
The procedures are listed in your virtual lab. You do not need to repeat them here. Please be sure to identify the test variable (independent variable), outcome variable (dependent variable).

Reminder: Test variable = the item you are changing or manipulating; Outcome variable = the item you are measuring

Test variable (independent variable):
Outcome variable (dependent variable):

Data:
Record the data from each location below.

Location Name Boundary Type
(C=Convergent, D=Divergent, or T=Transform) Year Observed
(5, 10, or 20 million years) Geologic Events Observed
(earthquakes, faults, ocean formation, mountains, volcanoes, island chains, seafloor spreading)
Location One
Himalayas 5 Event 1-
20 Event 2-
Location Two
East Africa 5 Event 1-
10 Event 2-
20 Event 3-
Location Three
San Andreas fault zone 5 Event 1-
10 Event 2-
20 Event 3-
Location Four
Aleutian Islands 5 Event 1-
20 Event 2-
Location Five
Mid-Atlantic Ridge 5 Event 1-
10 Event 2-
20 Event 3-
Location Six
Alpine Fault 5 Event 1-
20 Event 2-
Conclusion:
Your conclusion will include a summary of the lab results and an interpretation of the results. Please write in complete sentences.

What types of geological events or changes occur at divergent plate boundaries?
What types of geological events or changes occur at convergent plate boundaries?
What types of geological events or changes occur at transform plate boundaries?
Explain how these geological processes and interactions have changed Earth's surface through the years. Be sure to use evidence to support your answer.

Answers

Answer:

here are what i have so far, im doing this right now

Explanation:

Plate Tectonics Lab Report

Instructions: In the Plate Tectonic lab you will investigate the interactions between continental and oceanic plates at convergent, divergent, and transform boundaries around the globe. Record your observations in the lab report below. Y

Objective(s):  

To look at interactions between continental and oceanic plates, etc.  

Hypothesis:

In this section, please include the if/then statements you developed during your lab activity for each location on the map. These statements reflect your predicted outcomes for the experiment.

Location One: Select three events that you predict will be observed. If I explore two continental plates at a convergent boundary, then I will observe:

• earthquakes  

• mountains

• volcanoes

Location Two: Select three events that you predict will be observed. If explore two continental plates at a divergent boundary, then I will observe:  

• ocean formation  

• volcanoes

• seafloor spreading

you will submit your completed report

ps; you might want to change up the objective.

A geological event is a brief, spatially diverse, dynamic and ongoing occurrence in the history of the Earth.

What is geological event?

A geological event is a brief, spatially diverse, dynamic (diachronous), and ongoing occurrence in the history of the Earth that aids in the modification of the Earth system and the production of geological strata. The concept of event stratigraphy initially came up as a way to identify, analyse, and correlate how significant physical and biological events have affected the overall stratigraphical record.

Israel's Dead Sea basin Holocene sediments contain seismic activity. This can be considered a record of a geological event, an earthquake, that altered the strata. Geological events can occur over timescales of order of magnitude, from just a few seconds through millions of years, as well as on a variety of spatial scales, from the local to the globe.

1. Volcanoes and minor earthquakes

2. Volcanoes, earthquakes and fold mountains.

3. Earthquakes and fold mountains.

4. Magma from volcanoes is filled with nutrients that makes land fertile.

Therefore, a geological event is a brief, spatially diverse, dynamic and ongoing occurrence in the history of the Earth.

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SO3 is a covalent compound with no lone pairs on the central S atom. What's the structure of a molecule of SO3? Is an SO3 molecule polar or non-polar?
Question 14 options:

A)

Trigonal planar, non-polar

B)

Trigonal pyramidal, polar

C)

Trigonal planar, polar

D)

Trigonal pyramidal, non-polar

Answers

Answer:

A

Explanation:

it has non polar molecule, because it has the equal sharing of electrons, so equal repulsion from all sides. atoms are equally represented.

Answer:

Trigonal planar, non-polar

Explanation:

i need help ):

soo.. help me ! please (:

Answers

23 Sandwiches

Slices of cheese

6 slices of bread

0 slices of cheese

I think this is right.
Hope this helps :)

Answer:

23 Sandwiches

6 slices of bread

0 slices of cheese

Explanation:

brainliest pls:)

How many grams of CO2 are produced from 10 grams of C2H6

Answers

Answer:

Explanation:

no of moles of C2H6=10/30=0.33

complete combustion of 2 moles of ethane produce 4 moles of CO2 therefore 0.33 moles of C2H6 produced 0.66 moles of CO2

now moles =given mass/molar mass

mass= moles*molar mass

mass of CO2=0.67*44=29.48 g

what do y'all girls find attractive on a guy?

Answers

Answer:

alot of things

Explanation:

Which problem that the most scientists believe is partly caused by carbon dioxide?

Answers

Answer:

Climate change

Explanation:

Climate change is primarily a problem of too much carbon dioxide (CO2) in the atmosphere. This carbon overload is caused mainly when we burn fossil fuels like coal, oil and gas or cut down and burn forests

Mineral reaction to stress​

Answers

Answer:

Tenacity describes the reaction of a mineral to stress such as crushing, bending, breaking, or tearing. ... The majority of all minerals are brittle. An example is Quartz. (Minerals that are not brittle may be referred to as Nonbrittle minerals.)

Consider the following equilibrium.
NH3(aq) + H2O(l) equilibrium reaction arrow NH4+(aq) + OH −(aq)
What will happen to the equilibrium constant if the concentration of OH − increases through the addition of a small amount of NaOH(aq)?

Answers

Answer:

It will remain the same

Explanation:

The equilibrium constant of the reaction will remain the same if the concentration of the OH is increased.

Changes in concentration values of any of the reactant does not affect the value of the equilibrium constant.

The equilibrium constant is a constant at a given temperature. It is temperature dependent. Since we have no change in temperature, therefore, the equilibrium constant will not change.

The equilibrium constant if the concentration of OH − increases through the addition of a small amount of NaOH(aq) is :

- Remains same.

Equilibrium Reaction

The equilibrium constant of the response will stay the same in case the concentration of the OH is increased.

Changes in concentration values of any of the reactant does not influence the esteem of the equilibrium constant.

The equilibrium constant may be a consistent at a given temperature.

It is temperature dependent. Since we have no alter in temperature.

Learn more about "Equilibrium":

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Nose color is an inherited trait in dogs. Two puppies from the same set of parents have different color noses. One puppy has a pink nose and one puppy has a black nose. How can puppies from the same set of parents have different color noses?

A.
The pink-nosed puppy and the black nosed puppy must not actually be related.

B.
The pink-nosed puppy and the black nosed puppy inherited different combinations of the genes for nose color from their parents.

C.
The pink-nosed puppy has a different number of chromosomes than the black nosed puppy.

D.
The pink nosed puppy and the black nosed puppy have different chromosomes for the two nose colors.

Answers

Answer:

I thin its B im not sure though

Explanation:

The puppies from the same set of parents have different color noses because the pink-nosed puppy and the black nosed puppy inherited different combinations of the genes for nose color from their parents. Thus, the correct option is B.

What is a gene?

A gene is the unit of inheritance which is made up of DNA (deoxyribonucleic acid). Every person receives two copies or alleles of each gene which is inherited from each of the two parent.

Genes are made up of DNA, so each of the chromosome contains many different genes. Genes carry the information which determines the traits or characteristics that are passed on to their offspring or inherited from the parents.

The puppies from the same set of parents have different colored noses because pink-nosed puppy and the black nosed puppy inherited different combinations of the genes for nose color from their parents.

Therefore, the correct option is B.

Learn more about Inheritance here:

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How did the instrument in the picture help to disprove part of Dalton's atomic model?
A)
It showed all matter is made of atoms and that atoms are indivisible and
indestructible.
B)
It showed that all atoms of a particular element are not identical.
C)
It showed that compounds are formed by a combination of two or more
different kinds of atoms.
D)
It showed that a chemical reaction is a rearrangement of atoms.
No

Answers

Answer:

It’s b;)

Explanation:

Answer:

B is correct

Explanation:

which of the following is an example of
a physical property?

Answers

Answer:

physical property is property that you can touch, smell as it is bumpy it smells like eggs.

Explanation:

YW

Answer:

Physical properties include: appearance, texture, color, odor, melting point, boiling point, density, solubility, polarity, and many others.

Explanation:

8. Which object has molecules that are moving? water in a cup that is not moving, or water in the ocean
PLEASE HELP ASAP!!!

Answers

Answer:

Water in the ocean

Explanation:

The water in the ocean is always moving so all the molecules are was well

A 500.0-mL buffer solution is 0.100 M in HNO2 and 0.150 M in KNO2. Determine if each addition would exceed the capacity of the buffer to neutralize it.

a. 250 mg NaOH
b. 350 mg KOH
c. 125 g HBr
d. 1.35g Hl

Answers

Answer:

no one additions exceed the capacity of the buffer

Explanation:

given

Volume buffer = 500.0 mL = 0.5 L

mol HNO₂ = 0.5 L × 0.100 mol/L = 0.05 mol HNO₂

mol NO₂⁻ = 0.5 L × 0.150 mol/L = 0.075 mol NO₂⁻

solution

we know when any base more than 0.05 (HNO2) than exceed buffer capacity

and when any base more than 0.075 (KNO2) than exceed buffer capacity

when we add 250 mg NaOH (0.250 g)

than molar mass NaOH =40 g/mol

and mol NaOH = 0.250 g ÷ 40g/mol

mol NaOH  = 0.00625 mol

0.00625 mol NaOH will be neutralized by 0.00625 mol HNO₂

so it would not exceed the capacity of the buffer.

and

when we add 350 mg KOH (0.350 g)

than molar mass KOH =56.10 g

and mol KOH = 0.350 g ÷ 56.10 g/mol

mol KOH = 0.0062 mol

here also capacity of the buffer will not be exceeded

and

now we  add 1.25 g HBr

than molar mass HBr = 80.91 g/mol

and mol HBr = 1.25 g  ÷ 80.91 g/mol

mol HBr = 0.015 mol

0.015 mol Hbr will neutralize 0.015 mol NO₂⁻  

so the capacity will not be exceeded.

and

we add 1.35 g HI  

molar mass HI = 127.91 g/mol

so mol HI = 1.35 g ÷ 127.91 g/mol

mol HI = 0.011 mol

capacity of the buffer will not be exceed

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